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Importantly, the demonstrated acute in vitro inhibitory effects of low concentrations of bumetanide on islet insulin secretion (Sandstrom, 1990) seem to stem mostly from inhibition of NKCC1, as its exclusive elimination from β-cells precluded the effects of bumetanide ( However, experiments using islets of null mice lacking NKCC1 (NKCC1 KO ) gave unexpected results
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