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RNAscope in situ hybridization was performed on these sections using the RNAscope Multiplex Fluorescent Kit v2 (Advanced Cell Diagnostics) as per the manufacturer’s instructions, with a modification to the pre-treatment procedure (Protease IV incubation conducted for 20 min at room temperature) that allows for preservation of the fluorescent reporter signal while also providing optimal signal from the target mRNAs
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